Old Lady Possum was briefly featured in Emmet Otter's Jug-Band Christmas. She paid Emmet Otter and Wendell Porcupine $0.50 to mend the fence in front of her house.
A naked version of the puppet was later repurposed for The Muppet Show as the lead singer in "For What It's Worth" on episode 221.
Old Lady Possum later appeared during the "Rainbow Connection" finale of The Muppet Movie.
In the 2008 stage show adaptation, Old Lady Possum's full name is given as Penelope Possum. She was still a puppet, operated by Anney McKilligan.