- In The Muppets, the schedule for NBC shown in the CDE's office in The Muppets spoofs the show as Municipal Mom, in the Thursday night timeslot held by the show in the summer of 2010.
- In the season 6 episode, “London,” Jean-Ralphio compares his father's lawyer to Beaker from the Muppets and makes the meep sound that Beaker speaks with.
- In the season 7 episode, "The Johnny Karate Super Awesome Musical Explosion Show," Andy Dwyer (Chris Pratt) accidentally lets a dangerous spider loose in the studio while hosting a children's show, which leads to the network airing an on-air disclaimer. When slowed down and gone through frame-by-frame, it can be seen that halfway through the disclaimer, Dwyer briefly hijacks the message, saying "I had a dumb meeting with our dumb lawyer who was yelling at me for something I don't even know what it was he's so funny when he yells he looks like Beaker from the Muppets..."
- Aziz Ansari played Tom Haverford
- Fred Armisen played Raul in "Sister City" (2009)
- Will Arnett played Chris in "The Set Up" (2010)
- Kristen Bell played Ingrid de Forest (3 episodes, 2013–2014)
- Joe Biden played himself (2 episodes)
- Billy Eichner played Craig Middlebrooks (2013-2015)
- Jon Hamm played Ed (3 episodes, 2014-2015)
- John Hodgman played August Clementine in "Anniversaries" (2014)
- Rashida Jones played Ann Perkins
- Heidi Klum played Ulee Danssen in "London" (2013)
- Keegan-Michael Key played Joe (5 episodes, 2014–2015)
- Megan Mullally played Tammy Swanson (9 episodes, 2009-2015)
- Michelle Obama played herself
- Nick Offerman played Ron Swanson
- Patton Oswalt played Garth Blundin in "Article Two" (2013)
- Amy Poehler played Leslie Knope
- Richard Portnow played Mitch Savner (3 episodes, 2014)
- June Diane Raphael played Tynnyfer in "Doppelgängers" (2013)
- Paul Rudd played Bobby Newport (5 episodes, 2012-2015)
- Andy Samberg played Carl Lorthner in "Park Safety" (2010)
- Adam Scott played Ben Wyatt
- Peter Serafinowicz played Lord Edgar Covington in the two-parter "London" (2013) and "The Johnny Karate Super Awesome Musical Explosion Show" (2015)
- Jenny Slate played Mona-Lisa Saperstein (8 episodes, 2013-2015)
- Jorma Taccone played Roscoe Santangelo (3 episodes, 2015)
- Jeff Tweedy played the lead singer of Land Ho! (2 episodes, 2014)
- Henry Winkler played Dr. Sapirstein (9 episodes, 2013-2015)
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