Sherlock Holmes is a fictional detective created by Sir Arthur Conan Doyle and first published in 1887. He and his companion, Dr. Watson, have been spoofed or portrayed many times in Muppet productions, and the character Baskerville was named after the Holmes novel The Hound of the Baskervilles. Most of these portrayals rely heavily on the film characterization of Holmes by Basil Rathbone, including the familiar deerstalker cap and cape.
- Sherlock Hemlock from Sesame Street is a spoof of Holmes (and his dog Watson, a spoof of Dr. Watson). They even starred together in a series of segments entitled Mysterious Theater, which was a parody of Mystery!, a show that regularly featured Sherlock Holmes.
- Rowlf portrayed Holmes, with Baskerville as Watson in "Sherlock Holmes and the Case of the Disappearing Clues", the UK spot from episode 103 of The Muppet Show
- Baby Bunsen and Baby Beaker appeared as Sherlock Bunsen and Dr. Beaker in the Muppet Babies episode "The Muppet Broadcasting Company."
- Kermit appears as "Kermlock Holmes" in issue #13 of Muppet Babies Comics, with Fozzie as "Dr. Fozzie".
- Sherlock is the name given to Old Mother Hubbard's husband, who appears in standard Holmes garb and fancies himself a detective, in the "Mother Hubbard" installment of Mother Goose Stories
- Sir Arthur Conan Rubberducque, a fictional character who received an "as told to" credit for the book Sherlock Hemlock and the Great Twiddlebug Mystery is a reference to Holmes' creator, Sir Arthur Conan Doyle.
- In Ernie and Bert's Flashlight Adventure, one of the adventures they go on involve them dressed in Sherlock Holmes-like outfits while searching a castle.